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实验室如何进行清洁、消毒?

来源:http://www.sdrunjing.com/ 日期:2020-12-30 发布人:润静小编
PCR核酸检测生物安全实验室为二级生物安全防护实验室,一般用于开展新冠肺炎传染病检测及相关研究。
Novel coronavirus pneumonia laboratory is the two class biosafety laboratory, which is commonly used for the detection and research of new crown pneumonia infectious diseases. PCR
研究过程中由洗手盆、化学淋浴和高压灭菌器等设备排出或实验过程中产生的废液,如检测时接触的分泌物等,含有新冠病毒病原微生物,如不进行严格消毒灭菌处理,有可能污染实验环境,威胁实验人员健康。
The waste liquid discharged from the wash basin, chemical shower, autoclave and other equipment or generated during the experiment, such as the secretion during the detection, contains pathogenic microorganisms of neocoronavirus. If it is not strictly disinfected, it may pollute the experimental environment and threaten the health of the experimental personnel.
根据 GB19489-2008《实验室生物安全通用要求》和 RB /T199-2015《实验室设备生物安全性能评价技术规范》 要求,应科学处理污水,并对消毒效果进行检测。PCR核酸检测生物安全实验室废水处理主程序为高温加压方式灭菌,以化学消毒作为备用程序。化学消毒剂的使用方式有浸泡、冲洗、擦拭、喷洒和熏蒸等。
According to the requirements of GB 19489-2008 and GB 1589-2015, the laboratory disinfection effect should be evaluated. PCR nucleic acid detection biosafety laboratory wastewater treatment main program for high temperature and pressure sterilization, chemical disinfection as a backup program. The use of chemical disinfectants includes soaking, washing, wiping, spraying and fumigation.
                 山东实验室装修设计
含氯消毒剂对不锈钢、碳钢材料和铜质等金属材质腐蚀性较强,可考虑用作 PP、PVC 等材质排水管道、容器的消毒。
Chlorine containing disinfectant has strong corrosivity to stainless steel, carbon steel, copper and other metal materials, so it can be used for disinfection of PP, PVC and other drainage pipes and containers.
PCR核酸检测生物安全实验室中排水管道、活毒废水系统等设施设备管路长、结构复杂、体积很难准确估算,消毒验证时若使用芽孢悬液作为生物指示剂,存在芽孢用量大、配制困难、浓度不精确、难以控制风险等问题。相较于芽孢悬浮液作为生物指示剂,达到同样杀菌效果时,芽孢试纸条消毒时间更长、消毒剂浓度更高,更具挑战性。
In the laboratory of PCR nucleic acid detection, the drainage pipeline, live poison wastewater system and other facilities and equipment have long pipeline, complex structure, and difficult to accurately estimate the volume. If spore suspension is used as biological indicator in disinfection verification, there are some problems, such as large amount of spores, difficult preparation, inaccurate concentration, and difficult to control the risk. Compared with the spore suspension as a biological indicator, when the same germicidal effect is achieved, the sterilization time of the spore test strip is longer and the concentration of the disinfectant is higher, which is more challenging.
实验室生物安全作为实验室内一切活动的前提,安全、有效的消毒措施是实验室生物安全的重要保障。
Laboratory biosafety is the premise of all activities in the laboratory. Safe and effective disinfection measures are the important guarantee of laboratory biosafety.
SICOLABPCR核酸检测生物安全实验室在设计、建设过程中已经考虑了耐腐蚀性要求,对相关设施设备浸泡消毒建议选用消毒能力强的过氧乙酸和过氧化氢,尽可能降低生物风险。
The requirements of corrosion resistance have been considered in the design and construction of the biological safety laboratory for nucleic acid detection of sicolabpcr. It is recommended to select peracetic acid and hydrogen peroxide with strong disinfection ability for immersion disinfection of relevant facilities and equipment, so as to reduce the biological risk as much as possible.

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