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如何控制实验室的无菌环境?

来源:http://www.sdrunjing.com/ 日期:2020-08-04 发布人:润静小编
传统的空调系统,尤其是空调箱内部是细菌繁殖的良好温床,但是只要破坏或消除细菌滋生的条件,降低或抑制室内细菌发生,切断空调系统所有潜在的污染传播途径,就可以消除一切滋生菌的可能性。这与传统观念不同:解决微生物污染不是杀菌或除菌的方法问题,而是不让它产生。
The traditional air-conditioning system, especially the interior of the air-conditioning box, is a good breeding ground for bacteria. However, as long as the conditions for bacteria breeding are destroyed or eliminated, the occurrence of indoor bacteria is reduced or inhibited, and all the potential pollution transmission channels of the air conditioning system are cut off, the possibility of breeding bacteria can be eliminated. This is different from the traditional concept: to solve the problem of microbial pollution is not the method of sterilization or sterilization, but to prevent it from producing.
因此,应该将无菌空间看作一个控制体,对整个工艺操作,包括对设施、环境、物料与人员进行全过程控制,而不仅仅是对操作本身。在全过程消除一切可能产生微生物污染的隐患,才符合微生物控制的原理,才能为无菌环境控制提供更为有效的保障。全过程控制应包括进入控制体前控制、进入控制体后控制、退出控制体后的控制。
       实验室装备中常见的几种塑料特性
Therefore, aseptic space should be regarded as a control body to control the whole process of the whole process, including facilities, environment, materials and personnel, not just the operation itself. In the whole process to eliminate all possible microbial pollution hazards, only in line with the principle of microbial control, can provide more effective protection for aseptic environment control. The whole process control should include the control before entering the control body, the control after entering the control body and the control after exiting the control body.
进入前控制,即在进入控制体前应对人员、器械和材料等所有人与物进行严格消毒,并采用生物洁净技术对空气进行有效的过滤除菌,以保证进入控制体的空气无菌、无尘。由于无菌空间存在着许多发菌源,空间外的污染空间会随时随地渗透进来。要保持空间的无菌状态,进入后控制的应是对空间的污染以及人与人、人与物、物与物的交叉污染。
Before entering the control body, the personnel, instruments and materials should be strictly disinfected before entering the control body, and the air should be effectively filtered and sterilized by biological cleaning technology to ensure that the air entering the control body is sterile and dust-free. Because there are many bacteria sources in aseptic space, the polluted space outside the space will permeate in anytime and anywhere. In order to keep the sterile state of the space, the key points of the control after entering the space should be the pollution of the space and the cross pollution of people, people and things, and things and things.
对此,可通过采取生物洁净技术综合措施,如规划人流物流、合理建筑布局、消除发菌源的二次污染,降低交叉污染的隐患;设置气闸或缓冲、整个区域的梯度压差的控制,彻底阻止室外污染进入的可能;依靠室内气流技术,用大量低速空气以所需要的流型(乱流或单向流),稀释(或挤排)室内的所有发菌等,有效地维持无菌空间。退出后控制同样重要,如加强清场、清洁与处理等管理。只有及时将所有污物、废物等清出,并进行妥善处理,才有可能维持无菌空间及保护环境。
In this regard, we can take comprehensive measures of biological cleaning technology, such as planning the flow of people and logistics, reasonable building layout, eliminating the secondary pollution of bacteria source, reducing the hidden danger of cross pollution, setting air lock or buffer, controlling the gradient pressure difference of the whole area, so as to completely prevent the possibility of outdoor pollution from entering; Depending on the indoor airflow technology, a large amount of low-speed air is used to dilute (or squeeze) all the bacteria in the room with the required flow pattern (turbulent flow or unidirectional flow), so as to effectively maintain the sterile space. Post exit control is also important, such as strengthening the management of site clearing, cleaning and treatment. Only when all the dirt and waste are removed in time and properly treated can it be possible to maintain sterile space and protect the environment.

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